6 Questions 11 Answers 0 Followers
Questions related from Anthony Han Kang Tee
I have infected some mice virus, harvested their blood and aim to quantitate virus titer using real time pcr. May I know whole blood or serum sample is more suitable for this case? p/s: the...
25 April 2018 5,844 3 View
perhaps it can be determined in form of cell killing capacity or somehow other method?
12 May 2015 2,960 3 View
I am currently doing some virus infectious clones work.I have tried to in-vitro transcribe the coxsackievirus A16 (picornavirus) infectious clone to make rna, and then transfect them into HEK 293...
24 April 2015 8,641 8 View
I'm currently using two previously constructed polyclonal antibodies to test for the virus neutralization assay. Prior to the study, I tried immunofluorescence staining and both yield positive...
06 April 2015 2,820 6 View
I am doing a work constructing molecular clone (infectious clone) of virus and i wonder whether the length of the poly A tails will affects the replication of virus? i made it one with about 48...
11 February 2015 7,306 3 View
I have two fragments and a vector, first fragment is flanked by RE A and B, second fragment is flanked by RE B and C, and the vector is flanked by A and C. Now how am I going to clone them into...
17 December 2014 4,860 12 View