Anthony Han Kang Tee

5 Questions 10 Answers 0 Followers

Questions related from Anthony Han Kang Tee

Quantitate virus titer from mice blood. Whole blood or serum?

I have infected some mice virus, harvested their blood and aim to quantitate virus titer using real time pcr. May I know whole blood or serum sample is more suitable for this case? p/s: the...

25 April 2018 5,832 3 View

What happened to my electroporated infectious clone-derived virus RNA?

I am currently doing some virus infectious clones work.I have tried to in-vitro transcribe the coxsackievirus A16 (picornavirus) infectious clone to make rna, and then transfect them into HEK 293...

24 April 2015 8,572 8 View

Why is it my constructed antibodies don't work for virus neutralization assays?

I'm currently using two previously constructed polyclonal antibodies to test for the virus neutralization assay. Prior to the study, I tried immunofluorescence staining and both yield positive...

06 April 2015 2,800 6 View

Is length of poly A tails does a matter?

I am doing a work constructing molecular clone (infectious clone) of virus and i wonder whether the length of the poly A tails will affects the replication of virus? i made it one with about 48...

11 February 2015 7,294 3 View

How can I solve this cloning problem?

I have two fragments and a vector, first fragment is flanked by RE A and B, second fragment is flanked by RE B and C, and the vector is flanked by A and C. Now how am I going to clone them into...

17 December 2014 4,842 12 View