Why different protein activity from multiple codons for the same amino acid???

hii all i'm asking why multiple codons code for the same amino acid but different protein activity ? please attach papers or reviews thank you all zaynab

11 December 2018 2,673 5 View

"kit of pyruvate concentration" for serum samples are good or suitable for cell line suspension??

Have a nice day colleague i'm going to determine pyruvate concentration from cell line suspension (before and after treatment) for different duration times using Elabscience pyruvate assay kit...

09 October 2018 1,820 3 View

Is there protocol for restraining slids of immunohistchemistry?

04 May 2018 397 2 View

No title

03 April 2018 3,121 4 View

Iraq cancer registry 1990-2000??

have a nice day i'm very interested to study the statistic and gradual increase of cancer incidence in iraq specially between 1999-2000 year i search and couldn't find something i can depend just...

31 December 2017 6,408 1 View

MicroRNA from mesenchymal stem cell?

03 April 2016 3,439 3 View

How can i prepare skimmed milk?

03 April 2016 3,402 11 View

I have an extra band ... why?

02 March 2016 4,062 3 View

Any protocol for protein extraction?

02 March 2016 6,231 7 View

Is their any differences between ECL western blotting apparatus and gel band fluorescent detection apparatus?

31 December 2015 2,809 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Has anyone experienced a double band GAPDH in Western Blot?

I am using my GAPDH as a housekeeping protein for my Western Blot. For all the blots I have, my GAPDH was coming out as two bands. Therefore, I am using both bands for my analysis. Has anyone...

11 February 2021 6,376 3 View