Is it possible to used Flow Cytometry for diagnosis of pathogenic bacteria?

Bassam Yasein Khudaier @Bassam_Khudaier

12 December 2017 0 5K Report

We need a further tool like Flow Cytometry for accurate diagnosis or identification of pathogenic bacteria

Badges
Science method

More Bassam Yasein Khudaier's questions See All

Is the negative news notification affect human brain psychologically?

life is full of the bad and sad news that may affect people life

04 May 2019 5,247 4 View

To what extent is the boiling method acceptable in PCR and biotechnology applications?

Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology

09 October 2018 771 0 View

What is contig mapping?

DNA consist of series of regions coded for the whole genome.

05 June 2018 5,022 0 View

How is data mining useful to biotechnology research?

Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.

05 June 2018 5,222 0 View

What is the statement used to describe the process of synthesizing oligonucleotides directly on the glass slide?

There is a process used for synthesis oligonucleotides on the glass slide

05 June 2018 6,462 1 View

What makes a bone cell a bone cell as opposed to a liver cell lies largely in the set of genes that the cell expresses?

every somatic cell in the body has basically the same DNA.

04 May 2018 1,537 0 View

What is the role of Isoamyl alcohol in DNA isolation?

Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.

03 April 2018 8,853 2 View

What is the role of chloroform in DNA isolation?

chloroform is one of several chemical materials used in the extraction of DNA.

03 April 2018 3,713 2 View

What is the role of ethanol in DNA isolation?

one of the important steps in the extraction of DNA we use ethanol.

03 April 2018 3,969 0 View

What is the importance of proteinase K in DNA extraction ?

In the process of DNA extraction, we use proteinase K.

02 March 2018 4,725 5 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

13 July 2024 6,296 5 View