Is the negative news notification affect human brain psychologically?

life is full of the bad and sad news that may affect people life

04 May 2019 4,952 4 View

To what extent is the boiling method acceptable in PCR and biotechnology applications?

Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology

09 October 2018 705 0 View

What is contig mapping?

DNA consist of series of regions coded for the whole genome.

05 June 2018 4,777 0 View

What is the statement used to describe the process of synthesizing oligonucleotides directly on the glass slide?

There is a process used for synthesis oligonucleotides on the glass slide

05 June 2018 6,207 1 View

How is data mining useful to biotechnology research?

Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.

05 June 2018 4,974 0 View

What makes a bone cell a bone cell as opposed to a liver cell lies largely in the set of genes that the cell expresses?

every somatic cell in the body has basically the same DNA.

04 May 2018 1,441 0 View

What is the role of chloroform in DNA isolation?

chloroform is one of several chemical materials used in the extraction of DNA.

03 April 2018 3,370 2 View

What is the role of Isoamyl alcohol in DNA isolation?

Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.

03 April 2018 8,552 2 View

What is the role of ethanol in DNA isolation?

one of the important steps in the extraction of DNA we use ethanol.

03 April 2018 3,720 0 View

What is the importance of proteinase K in DNA extraction ?

In the process of DNA extraction, we use proteinase K.

02 March 2018 4,461 5 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View