is it possible to prepare PBS below 7.3 or I should work with sodium phosphate buffer which can be prepared below 7.3
The pKa of phosphate is ~7. At 2pH-units away from the pKa, the buffering capacity of a buffer is almost non-existent.
I want to synthesize porous silica nanoparticles (size range: 10nm to 100nm) using tetraethoxy orthosilicate (TEOS) in the presence of tween80 or CTAB as surfactant and liquid ammonia or NaOH. I...
03 April 2018 7,691 3 View
I have AuNPs modified with methyl mercapto-acetate and want to replace terminal methoxy group by peptide. I am using methyl mercapto-acetate as linker or you can suggest another linker to attach...
09 October 2015 9,442 2 View
I treated ovarian cancer cells with as prepared gold nanoparticles and peg modified for 24 hours. Then I collected the cell pellet by trypsinisation and centrifugation. Pellet with as prepared...
07 August 2014 6,472 8 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View