I have AuNPs modified with methyl mercapto-acetate and want to replace terminal methoxy group by peptide. I am using methyl mercapto-acetate as linker or you can suggest another linker to attach peptide. Thank in advance.
tiny volume of 10% glutaraldehyde may help, i am not sure. Attachment can be ascertained by FTIR analysis.
hey.. Suranjit.. thanks.. can you please provide link or reference paper if possible.. :)
is it possible to prepare PBS below 7.3 or I should work with sodium phosphate buffer which can be prepared below 7.3
04 May 2019 2,305 1 View
I want to synthesize porous silica nanoparticles (size range: 10nm to 100nm) using tetraethoxy orthosilicate (TEOS) in the presence of tween80 or CTAB as surfactant and liquid ammonia or NaOH. I...
03 April 2018 7,691 3 View
I treated ovarian cancer cells with as prepared gold nanoparticles and peg modified for 24 hours. Then I collected the cell pellet by trypsinisation and centrifugation. Pellet with as prepared...
07 August 2014 6,472 8 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?
03 August 2024 4,624 3 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View