How do we study nanoparticle-protein interaction using Raman spectroscopy? How its different from FTIR spectrosocpy?

We are well aware of FTIR spectroscopy and performed various experiments on protein conformational changes but never handle Raman spectroscopy. I want to know how could I extract more information...

04 May 2018 7,010 2 View

How to convert weight of nanoparticles (ug) into moles (uM)?

Molar concentration is used for homogeneous solutions but our nanoparticles are colloidal in nature. Nanoparticles are often different in sizes (10nm, 20nm, 100nm..). Then how could we convert...

11 December 2016 1,632 0 View

Can anyone help me with staining and quantification of glycosaminoglycans using Alcian Blue?

I want to check the purity of non-sulfated glycosaminoglycans through electrophoresis or titration method.

06 July 2016 6,670 4 View

What should be the initial inoculum for antimicrobial assays in a 96-well plate?

We are performing antimicrobial test using different antimicrobial agent. We treat bacteria at mid-log phase to detect the effectiveness of our antimicrobial agent. But we are not getting growth...

03 April 2016 6,407 8 View

Can anyone tell me how to conjugate a protein with nanoparticles and how to detect their sucessful conjugation?

Help me with some papers. Your help will be appreciated

10 November 2015 4,057 3 View

How can we quantify bacterial cell death on the base of membrane damage?

I want to compare the inhibition of bacterial cell death in presence of greenly synthesized nanoparticles and plant extracts. Both nanoparticles and plant extracts are showing the same ROS...

01 February 2015 2,158 8 View

How do fibrosarcoma HT1080 cells looks like in confocal DIC image?

I had performed nanoparticles cytotoxicity on these cell lines. I want to know if these cells are dead then how do they look like. Answers will be appreciable.

01 February 2015 7,239 6 View

How can we purify the nanoparticles synthesized from plant extracts which we later on use for interaction studies?

During interaction studies of nanoparticles with cellular components, I don't need any contamination of plant extracts.

03 April 2014 1,140 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View