How do we study nanoparticle-protein interaction using Raman spectroscopy? How its different from FTIR spectrosocpy?

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Is it possible to have 90% cell death through apoptosis as well as autophagy both at the same time?

My cell line is showing 90% cell death at a particular drug concentration. Can it be possible that apoptosis and autophagy play role at the same time?

06 July 2018 3,990 3 View

How to convert weight of nanoparticles (ug) into moles (uM)?

Molar concentration is used for homogeneous solutions but our nanoparticles are colloidal in nature. Nanoparticles are often different in sizes (10nm, 20nm, 100nm..). Then how could we convert...

11 December 2016 1,632 0 View

Can anyone help me with staining and quantification of glycosaminoglycans using Alcian Blue?

I want to check the purity of non-sulfated glycosaminoglycans through electrophoresis or titration method.

06 July 2016 6,670 4 View

What should be the initial inoculum for antimicrobial assays in a 96-well plate?

We are performing antimicrobial test using different antimicrobial agent. We treat bacteria at mid-log phase to detect the effectiveness of our antimicrobial agent. But we are not getting growth...

03 April 2016 6,407 8 View

Can anyone tell me how to conjugate a protein with nanoparticles and how to detect their sucessful conjugation?

Help me with some papers. Your help will be appreciated

10 November 2015 4,057 3 View

How can we quantify bacterial cell death on the base of membrane damage?

I want to compare the inhibition of bacterial cell death in presence of greenly synthesized nanoparticles and plant extracts. Both nanoparticles and plant extracts are showing the same ROS...

01 February 2015 2,158 8 View

How do fibrosarcoma HT1080 cells looks like in confocal DIC image?

I had performed nanoparticles cytotoxicity on these cell lines. I want to know if these cells are dead then how do they look like. Answers will be appreciable.

01 February 2015 7,239 6 View

How can we purify the nanoparticles synthesized from plant extracts which we later on use for interaction studies?

During interaction studies of nanoparticles with cellular components, I don't need any contamination of plant extracts.

03 April 2014 1,140 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View