Can anyone help me with staining and quantification of glycosaminoglycans using Alcian Blue?

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Nabin Poudel

Kamaya biological has nice kit for detection of sulfated gags using alcian blue.

Greg Cote

The attached paper by Cowman ought to help.

Parth Sarathi Nayak

Thanks Greg Cote but these protocols are only related to sulfated-GAGs not to the non-Sulfated GAGs.

As the Ramus paper explains, Alcian Blue is a cationic dye that interacts with anionic polymers, including both sulfated and carboxylic-acid containg polysaccharides. If your polysaccharide is polyanionic, then it should work the same. If your polysaccharide is not polyanionic, then you need to choose a different method. The periodate-Schiff method is good for most glycans.