Can anyone help me with staining and quantification of glycosaminoglycans using Alcian Blue?

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Parth Sarathi Nayak

Thanks Greg Cote but these protocols are only related to sulfated-GAGs not to the non-Sulfated GAGs.

Greg Cote

As the Ramus paper explains, Alcian Blue is a cationic dye that interacts with anionic polymers, including both sulfated and carboxylic-acid containg polysaccharides. If your polysaccharide is polyanionic, then it should work the same. If your polysaccharide is not polyanionic, then you need to choose a different method. The periodate-Schiff method is good for most glycans.