Parth Sarathi Nayak

9 Questions 18 Answers 0 Followers

Questions related from Parth Sarathi Nayak

Is it possible to have 90% cell death through apoptosis as well as autophagy both at the same time?

My cell line is showing 90% cell death at a particular drug concentration. Can it be possible that apoptosis and autophagy play role at the same time?

07 July 2018 4,045 3 View

How do we study nanoparticle-protein interaction using Raman spectroscopy? How its different from FTIR spectrosocpy?

We are well aware of FTIR spectroscopy and performed various experiments on protein conformational changes but never handle Raman spectroscopy. I want to know how could I extract more information...

05 May 2018 7,123 2 View

How to convert weight of nanoparticles (ug) into moles (uM)?

Molar concentration is used for homogeneous solutions but our nanoparticles are colloidal in nature. Nanoparticles are often different in sizes (10nm, 20nm, 100nm..). Then how could we convert...

12 December 2016 1,701 0 View

Can anyone help me with staining and quantification of glycosaminoglycans using Alcian Blue?

I want to check the purity of non-sulfated glycosaminoglycans through electrophoresis or titration method.

07 July 2016 6,722 4 View

What should be the initial inoculum for antimicrobial assays in a 96-well plate?

We are performing antimicrobial test using different antimicrobial agent. We treat bacteria at mid-log phase to detect the effectiveness of our antimicrobial agent. But we are not getting growth...

04 April 2016 6,466 8 View

Can anyone tell me how to conjugate a protein with nanoparticles and how to detect their sucessful conjugation?

Help me with some papers. Your help will be appreciated

11 November 2015 4,115 3 View

How do fibrosarcoma HT1080 cells looks like in confocal DIC image?

I had performed nanoparticles cytotoxicity on these cell lines. I want to know if these cells are dead then how do they look like. Answers will be appreciable.

02 February 2015 7,308 6 View

How can we quantify bacterial cell death on the base of membrane damage?

I want to compare the inhibition of bacterial cell death in presence of greenly synthesized nanoparticles and plant extracts. Both nanoparticles and plant extracts are showing the same ROS...

02 February 2015 2,208 8 View

How can we purify the nanoparticles synthesized from plant extracts which we later on use for interaction studies?

During interaction studies of nanoparticles with cellular components, I don't need any contamination of plant extracts.

04 April 2014 1,186 1 View