Is it possible to do immunohistochemistry on brains injected with Evans blue?

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Robert Bronstein

Use GFAP, stains the glial scar well.

Yun Li

yes, but would the glial scar show up as early as 24h into repercussion? I want to do different time periods, starting at 24h and going up to 7d

Catherine A Gorrie

Are you using DAB or Flourescent? You could counterstain nuclei blue with Hoechst or similar under flourescent light This should allow you to identify the damaged infarct area, certainly by a few days. Alternatively stain an adjacent sections with H&E or Nissl stain and overlay on your IHC images.

Umeo Ito

Evans blue is combined with albumin in the blood vessels, and leak out through the disrupted BBB. This indicate the site of disrupted BBB. However, it dose not always indicate the precise location and extension of infarction.

Thanks for all the answers, it was just a thought. Wouldn't it be interesting to see what proteins/markers would be increased in the areas with disrupted BBB? I never met to see to the precise location and extension of infarct, there's plenty of other ways to do that. I wanted to see factors that contribute towards BBB disruption.