7 Questions 16 Answers 0 Followers
Questions related from Yun Li
So adding mounting medium and then placing the coverslip onto the slide sounds and looks easy enough, but there's still so many things that could go wrong. For one, I just can't seem to be able to...
08 August 2018 782 6 View
I saw this phrase used in a guy's C.V. and I've never really heard of it before? I presume it's swedish, since the guy came from Karolinska Institute. What's the equivalent of this position in...
03 March 2016 1,817 1 View
My lab recently purchased a monoclonal antibody of the peptide Galanin from Sigma-Aldrich. According to the user's manual, the band should be found at about 38kD. The resultant Western blot...
12 December 2015 7,109 9 View
Quick question, can the commercially available staining method Diff-Quik be used to determine the extent of damage in brain slices from MCAO mice? It seems like a really great way to stain cells,...
05 May 2015 7,004 3 View
As the question suggests, I would like to know why glycerol can't be used on DAB stained slices. There's also something that's always been bugging me. I used a Roche TUNEL staining kit earlier and...
10 October 2014 839 3 View
I would like to see the localization of a specific protein on the peri-infarct region of mice brains that went through ischemia/reperfusion. But it's pretty hard to see the infarction area during...
06 June 2014 3,024 5 View
I'm trying to work through the protocols for TUNEL staining using In situ Cell Death Detection Kit (Roche), and the recipe of the permeabilization solution keeps bugging me. I admit, we never used...
03 March 2014 6,554 8 View
