Does anyone know any good tips and tricks for avoiding air bubbles in coverslipping IHC slides?

So adding mounting medium and then placing the coverslip onto the slide sounds and looks easy enough, but there's still so many things that could go wrong. For one, I just can't seem to be able to...

07 August 2018 690 6 View

Would a monoclonal antibody created from GST tagged full-length recombinant protein have higher molecular weights than the original protein?

My lab recently purchased a monoclonal antibody of the peptide Galanin from Sigma-Aldrich. According to the user's manual, the band should be found at about 38kD. The resultant Western blot...

11 December 2015 7,077 9 View

Can Diff-Quik be used to determine the extent of cerebral damage in MCAO?

Quick question, can the commercially available staining method Diff-Quik be used to determine the extent of damage in brain slices from MCAO mice? It seems like a really great way to stain cells,...

04 May 2015 6,973 3 View

Why can't you use glycerol to seal DAB stained samples?

09 October 2014 803 3 View

Is it possible to do immunohistochemistry on brains injected with Evans blue?

I would like to see the localization of a specific protein on the peri-infarct region of mice brains that went through ischemia/reperfusion. But it's pretty hard to see the infarction area during...

05 June 2014 2,988 5 View

What is sodium citrate for in permeabilization solutions?

I'm trying to work through the protocols for TUNEL staining using In situ Cell Death Detection Kit (Roche), and the recipe of the permeabilization solution keeps bugging me. I admit, we never used...

02 March 2014 6,452 8 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

Database of European Football Players Salaries ?

Hello everyone, I am interested in writing about a dissertation about the wages of the football players of the 5 main leagues in Europe. I wanted to know if someone had an up to date data base...

17 February 2021 8,847 3 View