Does anyone know any good tips and tricks for avoiding air bubbles in coverslipping IHC slides?

So adding mounting medium and then placing the coverslip onto the slide sounds and looks easy enough, but there's still so many things that could go wrong. For one, I just can't seem to be able to...

07 August 2018 690 6 View

What kind of position is "senior forskare"?

I saw this phrase used in a guy's C.V. and I've never really heard of it before? I presume it's swedish, since the guy came from Karolinska Institute. What's the equivalent of this position in...

02 March 2016 1,781 1 View

Would a monoclonal antibody created from GST tagged full-length recombinant protein have higher molecular weights than the original protein?

My lab recently purchased a monoclonal antibody of the peptide Galanin from Sigma-Aldrich. According to the user's manual, the band should be found at about 38kD. The resultant Western blot...

11 December 2015 7,077 9 View

Why can't you use glycerol to seal DAB stained samples?

09 October 2014 803 3 View

Is it possible to do immunohistochemistry on brains injected with Evans blue?

I would like to see the localization of a specific protein on the peri-infarct region of mice brains that went through ischemia/reperfusion. But it's pretty hard to see the infarction area during...

05 June 2014 2,988 5 View

What is sodium citrate for in permeabilization solutions?

I'm trying to work through the protocols for TUNEL staining using In situ Cell Death Detection Kit (Roche), and the recipe of the permeabilization solution keeps bugging me. I admit, we never used...

02 March 2014 6,452 8 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View