As the question suggests, I would like to know why glycerol can't be used on DAB stained slices.
There's also something that's always been bugging me. I used a Roche TUNEL staining kit earlier and the manual specifically said that glycerol can be used to seal the cover slips before looking at the results on a microscope, but the end product was stained by DAB. So was the manual wrong to say that, or is it just not something to be concerned about?
I would like to thank anybody who looks and answers this question beforehand; this is a great place to get answers.
You can coverslip with glycerol once DAB is precipitated, you won't have any problem with the staining as long it is insoluble in water based mediums. But keep in mind that overtime the preparation will deteriorate (dry, fungi etc).
Dehydration + xylene + DPX is the common choice for DAB staining because you can store for a long time among other advantages.
It depends on your aims. Glycerol alone as mounting medium does not harden and will dry out over time. So for longer storage you have to seal the egdes with something like Eukitt. This can be helpful, if you work with chromogens, that are soluble in organic solvents and would disappear in xylene-baths. For chromogens, that are insoluble in organic solvents, it would be better to take the way over dehydration, clearing and mounting in Eukitt or similar. On the other side, if you don't store your preparation, you can choose what you like ;-)
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