I am looking for methods by which I can knock down the expression a protein in acute hippocampal slice culture using siRNA/shRNA or any other methods. First of all, is it possible to do gene knock out using siRNA/shRNA in acute hippocampal slice culture? If yes then please tell me how to go ahead for that. If not, what are the alternative methods to do so? I already done with a pharmacological inhibitor for this purpose but I want to do knockdown based experiments to prove strongly. Please kindly give me your suggestions.