Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

I was running Gaussian 09W Programmer in Frequency analysis but Chk File is not opened ?

gaussian frequency analysis was completed but chk file not open, Alpha MO energies Missing in view of the display.

24 January 2021 1,529 2 View

How to obtain the mobility from the EIS data?

Electrochemical impedance spectroscopy

09 August 2020 7,608 2 View

The resistance of the sensor was decreased after doping at room temperature why?

the resistance of the sensor was decreased after doping at room temperature why

18 June 2020 5,636 2 View

Hi, I would like to sense the Fe(III) in water, therefore how can I overcome the pH interference?

when adding the Fe compound in the water it gets deprotonated and from strong acid. 1.adding base leads to salt formation 2.adding buffer solution leads to Fe concentration change.

11 March 2020 1,785 3 View

Can anyone please suggest some curent research topics in Concrete Technology ?

concrete technology, materials

10 November 2019 8,423 12 View

Whether soil inorganic carbon sequestration leads to land degradation?

Dear senoirs friends, We know that carbon sequestrated as soil organic and inorganic . Several fancy literature reported that IC is high than OC. The organic forms usually labile and available...

22 March 2019 5,606 3 View

NF Kappa B knocked out cell line?

Can anyone please suggest a human cell line with NFkappaB knock out? Will be using it as a control for my experiment. Thanks in adavance.

08 November 2018 9,232 2 View

Can I use Laser Displacement Sensor (LDS) to measure strain?

I am building a small scale UTM machine. I have placed a load cell to measure stress. Can I use a LDS(Laser Distance Sensor) to measure displacement which can be ultimately used to measure strain?

22 April 2018 3,192 1 View

Whether non-significant data represented in chart or graphical representation?

22 September 2017 6,417 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View