4 Questions 7 Answers 0 Followers
Questions related from Samidurai Manikandan
I have isolated the Triton insoluble protein aggregates from the cell culture media and blood serum. I got the pellet (insoluble protein) in the final stage of the isolation process and it’s not...
22 March 2023 9,941 10 View
Hello all, I would like to express a gene of full-length human Tau (2N4R) into BL21 DE3+ strain of E. coli. I have sequenced the plasmid(pET15b) and it has the right sequence. The plasmid...
04 December 2022 753 2 View
I am working in the neurovegetative diseases. I want to isolate the extracellular protein aggregates from the cell culture media, serum, and Plasma. I have done it with cells and tissues before,...
06 September 2022 1,399 0 View
Hello Everyone, I am looking to purify Recombinant tau monomers for my studies. But we don't have FPLC system with us. I am wondering any methods available to purify without the FPLC system....
26 August 2022 3,381 7 View