Is it possible that Hydrocortisone turn into crystals after stock frozen due to the high concentration?

12 September 2013 1 6K Report

I'm using hydrocortisone to culture murine cells. The stock I use is 50 μg/ml. I store it at -20°C in small aliquots. However, I often found crystals in the media under microscope. Has anyone had such a problem? How do you store hydrocortisone?

Fei Yang

I found crystals in diluted medium. The medium i use is α-MEM, supplemented with 20% FCS. And the stock was also prepared in this medium. Is it possible that FCS turns into crystal under this condition? And another problem, some paper said that they always use medium freshly added with HC. How long is HC stable in complete medium? Can I add it to 100 ml medium and use it up within two weekls? Thanks a lot!

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