Fei Yang

10 Questions 3 Answers 0 Followers

Questions related from Fei Yang

How can I prove that the microparticles in a tissue section are apoptotic bodies?

I want to prove the microparticles in the tissue section are apoptotice bodies. I've searched on the internet. some told me that TUNEL staining could do that. However, I think TUNEL staining can...

04 September 2014 730 2 View

Are there any protocols for inducing immune tolerance to immunogenic protein in mice?

I am interested in immune tolerance. Are there any experimental protocols for incuding immune tolerance to an immunogenic protein? Thanks for your help!

28 August 2014 5,439 1 View

Why is chichen ovalbumin chosen as one of the most popular model antigen?

there are millions of proteins are immunogenic, however there are only several used as model antigens in immunological studies. One of the best known is chicken ovalbumin. Could someone tell me...

26 August 2014 4,649 9 View

Is there any anaesthetic that can be used in place of ketamine on mice?

We have been using Ketamine + Xylazine as a standard anaesthetic for mice in our lab. However, due to some policy problems, ketamine is no longer produced here in China. I wonder if there is any...

23 February 2014 2,133 9 View

What's the size of plasmid DNA in nanometers?

I want to enclose a 6 kbp plasmid DNA into nanoparticles. But the nanoparticles I use is about 30 nm in diameter. I wonder whether they can hold the DNA. Can someone give some advice? Thanks!

02 January 2014 3,987 1 View

Is it possible that Hydrocortisone turn into crystals after stock frozen due to the high concentration?

I'm using hydrocortisone to culture murine cells. The stock I use is 50 μg/ml. I store it at -20°C in small aliquots. However, I often found crystals in the media under microscope. Has anyone had...

12 September 2013 6,053 1 View

Can I use a different cell lines as negative or positive controls when doing flow cytometry?

When doing Flow Cytometry, I used B16 cells as negative control of IFN-gamma and Granzyme B. But apparently, the sizes of B16 and lymphocytes are so different. Is this allowed? Or, I can only use...

09 May 2013 8,393 3 View

How can I culture purified NK cells in vitro for a long time, for example 1-2 weeks?

I'm culturing murine NK cells in vitro. However, the second day post isolation, 80% or more of the cells are dead or apoptotic. The medium I used was a-MEM supplemented with 10% FBS, 10 ng/ml...

07 April 2013 8,126 8 View

Can murine FcR recognize rat antibodies?

I'm reading a paper about ADCC. The authors used rat antibodies to coat tumor cells. But the NK cells they used were from mice. Can murine FcR recognize rat antibody Fc ends? I don't know. Could...

21 February 2013 7,019 3 View

How can I precoat a petri dish with antibodies by myself?

I want to deplete some subpopulations of immune cells from bone marrow with petri dish precoated with antibodies. But I don't know how to make it and how much antibody I need. Can anyone who has...

24 January 2013 4,454 14 View