Or I have to freeze in -80 after removing the RNAlater fluid?
Thank you all,
Shani
and another thing. I allready put my samples in -80 in Rnalater fluid directly after harvest.
Is that ok? Should I put it in 4 degrees before Rna extraction?
I understand your point. I have no experience with freezing samples in RNALater at -80 as I usually put them in RNAlater and incubate at 4 C overnight so it can go inside the samples, then it could be frozen at -20. But maybe next time just freeze them in -80 without the need to RNALater.
We keep our tissue in RNAlater, store it at -80C for months or years (with RNAlater), then thaw the sample on ice when we need to homogenize and extract RNA. We get RNA quality good enough for RNA-seq.
Hello Dear Shani,
Did you get good RNA extraction results when you store your samples directly @-80 without +4 incubation?
please it is possible to keep your RNA samples at -20 C for more than a week before transfering them at -80 C and at the end you get the desired yields for your analyses.
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