Shani Dror

7 Questions 9 Answers 0 Followers

Questions related from Shani Dror

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

19 July 2024 5,183 4 View

How to grow intratestinal macrophages in culture?

Hi, Im trying to isolate intratestinal macrophages from mouse lungs, and grow them in culture for a few days, to collect secretome. Does somebody have a good protocol for it? I tried face...

12 May 2022 1,011 3 View

How to measure rRNA amount?

Hi all, I try to figure out weather there is a change in my rRNA following treatment. when I treat the cells (mouse cells) and isolated RNA, I see a major difference in RNA concentration, without...

07 September 2018 812 7 View

Is it ok to keep my tissue in RNAlater and put in -80?

Or I have to freeze in -80 after removing the RNAlater fluid? Thank you all, Shani

01 April 2017 4,226 6 View

Anyone knows protocol for viva spin tubes?

Hi all, I want to do western blot for one of my secretome proteins. I thought to use viva spin in order to concentrate the medium.How should I proceed? can I add lysis buffer? how much?or should I...

25 July 2016 6,024 3 View

Staining cell after FACS sorting?

Hi, I want to sort fibroblast from a mice, than fix them and stain it for immunofluorescence. I dont want to culture it first. I have no experience with that. What is the best way to do it?

23 December 2015 4,583 3 View

Could anyone suggest a dye used in flow cytometry that can stain specifically to RNA but not DNA?

I want to show RNA delivery between cells using stained RNA.

10 September 2015 5,200 3 View