Uzma Din

2 Questions 3 Answers 0 Followers

Questions related from Uzma Din

U6 snRNA control in qRT-PCR for miRNA?

Is it supposed to be added along with my miRNA primer in the same pool of RNA? Lets suppose I am checking the expression of mirX microRNA and I have 2 conditions- treated and non-treated. So per...

05 April 2017 5,463 3 View

I use Ca-Mg free PBS for brain dissection in my primary culture experiment. Should I add any antibiotic in this buffer too?

I use 50ug/ ml Gentamycin in my culture media already. I have to dissect the sub-ventricular zone under a microscope which takes a while to do and all the steps upto the collection of region of...

27 July 2016 6,898 2 View