Are there Hif1a antibodies that work reliably for immunofluorescence on frozen sections?

A few years back I tried several available Hif1a antibodies on frozen sections of heart and lung. None of them worked very well. Most worked well for immunoblots. Have there been any advances in...

12 October 2020 7,727 2 View

Is there a program/code for distinguishing between plants in thermal camera imaging?

My research includes monitoring canopy temperature over time with a thermal FLIR camera. I am looking for a code/program that will help me analyze the videos easily and automatically. For my...

24 February 2020 4,722 3 View

How to measure rRNA amount?

Hi all, I try to figure out weather there is a change in my rRNA following treatment. when I treat the cells (mouse cells) and isolated RNA, I see a major difference in RNA concentration, without...

06 September 2018 724 7 View

Anyone knows protocol for viva spin tubes?

24 July 2016 5,940 3 View

Could anyone suggest a dye used in flow cytometry that can stain specifically to RNA but not DNA?

I want to show RNA delivery between cells using stained RNA.

09 September 2015 5,115 3 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

Absorption behaviour of anechoic model?

I have modelled a unit-cell of an anechoic structure using ANSYS. The layer is silicone and has been assigned as an acoustics physics region, with an air cavity inside (the air has been modelled...

18 February 2021 4,119 1 View

Can you suggest a good domain identification tool?

I am working on a project to identify sensitive information from text and I am looking into the below categories: -adult content -health -politics -crime Can someone suggest an open source tool...

16 February 2021 4,844 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

What is the appropriate role of memorization in foreign language teaching?

Research in FLT has always been concerned with the role of memory in learning and the relationship between memorization and learning.

04 February 2021 2,202 5 View