How to measure rRNA amount?

Hi all, I try to figure out weather there is a change in my rRNA following treatment. when I treat the cells (mouse cells) and isolated RNA, I see a major difference in RNA concentration, without...

06 September 2018 724 7 View

Failed expression of recombinant nucleation peptide, due to a short spacer between T7 promoter to RBS?

hi all, i'm having a problem to express my gene in E.Coli (BL21) of a nucleation peptide. nowadays i'm suspecting that it might be because of a too short spacer in my vector: (the "map", and the...

27 June 2018 3,214 5 View

Why I get no amplification using degenerative PCR?

Hello, I'm trying to amplify a gene in both eDNA and bacterial DNA (a strain of Bacillus, that should be the positive control) using degenerative primers, their sequence was published in a...

10 December 2017 503 3 View

What are the best bone marrow derived osteocytes and chondrocytes flow cytometry markers?

I am trying to identify by flow cytometry osteogenic and chondrogenic lineages differentiated from MSCs in human BM. What would be the best markers (intracellular or extracellular) for this...

27 September 2017 7,371 3 View

Anyone knows protocol for viva spin tubes?

24 July 2016 5,940 3 View

Purifying 1000 cells from 1.5ml PBS, any ideas?

Hi all, I'm looking for ideas to purify 1000 cells from 1.5ml PBS while minimizing cell loss and as clean as possible (not using beads for example). Also standard centrifugation is not an option...

17 July 2016 2,548 3 View

Can anyone recommend culture conditions for human blood primary dendritic cells?

medium composition, density, anything alse?

12 January 2016 8,909 2 View

Any recommendations for a good antibody against BDNF?

Hi I'm looking for a good antibody anti BDNF to use in Western blot and IHC to characterize BDNF in the mouse brain. Preferably, an antibody that works good for both applications would be...

01 November 2015 3,347 6 View

Could anyone suggest a dye used in flow cytometry that can stain specifically to RNA but not DNA?

I want to show RNA delivery between cells using stained RNA.

09 September 2015 5,115 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Can we proceed to perform Two-way Mixed-ANOVA despite normality violations?

While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...

24 February 2021 228 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View