I have read quite a few articles and papers which say how importanrt it is to do a standard curve when setting up a new qPCR experiment. However, I have also read that this is more applicable when using SYBR green and less for probe based experiments. I am using TaqMan probes and I am unable to find a clear answer as to whether or not it is neccesary to perfrom a primer efficiency standard curve . Also I use a predesigned primer/prober mix. Could someone please suggest whether on not a standard curve is necessry in this case. Thank you

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