When we use transfected cell lines for xenografts (such as for GFP-tagging) we make sure that they are stably-transfected (https://altogen.com/services/generation-stably-expressing-cell-lines-28-days/). Transient transfection is not sufficient for xenografting as the study runs sometimes for more than a month, at which point the initial changes in gene expression are no longer present.

Hi Muhd,

Two common breast cancer cell lines that are used (based on the literature) are MCF7 and MCF10AT cells. In our lab we typically choose a cell line that is sensitive to our compound in cell based assays. For example if you are interested in targeting HER2, you could use a HER2 amplified or mutated cell line.

Transfected cells are commonly used in xenograft models, but the cell lines should be stably transfected. It is common for us to stably transfect an inducible shRNA construct into the cell line. After the xenograft has been established we induce shRNA expression, and then observe the effect of knockdown of our gene on xenograft growth.

Transiently transfected cells will not maintain the transfected plasmid as the cells proliferate in a xenograft. To ensure all the cells contain the trasfected gene/cDNA /shRNA it is highly referred to develop stable cell lines/clones and use the best characterized one for implantation.

When we use transfected cell lines for xenografts (such as for GFP-tagging) we make sure that they are stably-transfected (https://altogen.com/services/generation-stably-expressing-cell-lines-28-days/). Transient transfection is not sufficient for xenografting as the study runs sometimes for more than a month, at which point the initial changes in gene expression are no longer present.