Muhd S

5 Questions 7 Answers 0 Followers

Questions related from Muhd S

What are the things we have to look out for or consider after implanting estrogen pellets in NSG mice?

I have had experience implanting estrogen pellets (from Innovative Research of America) in nod-scid mice and at week 4 onwards, some form of toxicity and mortality can be observed. My experiments...

08 August 2014 8,906 2 View

Is it a "must"/"highly recommended" for transduced cell lines to be used for xenograft models instead of the usual transfected cells?

For example, breast cancer cells in mice models. Can transfected cells be used (assuming the transfection efficiency is high)?

01 January 2013 4,618 4 View

If my transduction efficiency is high enough, can the transduced cells be used for xenograft implantation without the need for subcloning, etc.?

More information: The transduced sequences include GFP and antibiotic resistance. Or, can I simply subject the cells to a period of antibiotic selection and then harvest them right away for...

01 January 2013 6,454 6 View

Cells virally transduced with miRNA "tagged" with GFP but RT-PCR could not detect the transduced miRNA

I have been transducing MB-231 cells with lentiviruses carrying a GFP-miRNA construct. The cells showed good GFP fluorescence but RT-PCR does not show any overexpression of the miRNA. RT-PCR has...

01 January 2013 2,740 11 View

Using the BLOCK-iT™ Pol II miR RNAi Expression Vector Kit from Invitrogen

According to the protocol, when designing the pre-miRNA double-stranded DNA oligo, the mature miRNA sequence used should be 21 nucleotides long (You may refer to Page 16 of the protocol here:...

12 December 2012 7,223 3 View