I have had experience implanting estrogen pellets (from Innovative Research of America) in nod-scid mice and at week 4 onwards, some form of toxicity and mortality can be observed. My experiments involve MCF-7 xenografts. I am moving on to NSG mice as they are better models for metastasis....hence I am wondering if the same problems (bladder stones etc) still apply to NSG mice.
Also, I have ever read a paper mentioning that estrogen supplementation is not necessary for MCF-7 growth in NSG mice... anyone can confirm this?
Thank you very much in advance!
Hello! My experience with .18mg 17beta 60 day release estrogen pellets from Innovative Research of America has been fine, no side effects nor toxicity, including in NSG mice, even beyond week 4 for slower growing tumors. The only issue we have seen is sometimes the pellet does not dissolve completely, but the tumor has still grown so the pellet must have still been releasing the estrogen. Our general rule is to implant estrogen pellets for any ER+ cell lines or tumors, even in NSG mice. Good luck!
Supraphysiological levels of E2 can provoke anorexia, so keep an eye on food intake and weight loss.
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