I am working on extracting RNA from the tissue of mice Thymus for sequencing using the Trizol method. Upon doing a quality inspection with a Quibit and a tape station however, I notice that there is contamination of DNA, and smaller amounts of RNases. I know that glycogen is soluble in chloroform, and since during the Trizol method you add isopropanol to the solution to begin isolation, would this be an ideal step to add glycogen? There would be roughly a 1:1 ratio of the chloroform RNA solute and the Isopropanol.