Is Cas12a (Cpf1) more efficient that SpCas9 for HDR-mediated editing?

Cristina Gutierrez Caballero @Cristina-Gutierrez-Caballero

05 May 2020 0 5K Report

Hi everybody,

Cas12a (Cpf1) has been proposed to be more efficient for HDR-mediated genome editing than SpCas9 as it produces a staggered cut with a 5′ overhang. What is your experience with this nuclease for generating knock-ins?

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