Cristina Gutierrez Caballero

3 Questions 5 Answers 0 Followers

Questions related from Cristina Gutierrez Caballero

Which synthetic gRNA would you recommend?

Hi, I am planning to use RNP nucleofection to insert a DNA fragment by HDR-mediated editing by CRISPR-Cas9. I have always used dual promoter plasmids for this, so I don't have experience with the...

05 May 2020 3,947 8 View

Is Cas12a (Cpf1) more efficient that SpCas9 for HDR-mediated editing?

Hi everybody, Cas12a (Cpf1) has been proposed to be more efficient for HDR-mediated genome editing than SpCas9 as it produces a staggered cut with a 5′ overhang. What is your experience with...

05 May 2020 4,745 0 View

Is ssDNA-mediated HDR effective for knock-in insertions of 50-100 bp?

Hi, I am going to use CRISPR nCas9 to insert 80bp in a specific region of the genome (in HEK293T). I am aware that the use of ssDNA as template is recommended for insertions

11 December 2018 506 6 View