Is BamH1(not hf) compatible with cutsmart buffer?

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Dominique Liger

Hi there,

According to NEBcloner, both enzymes are 100% active in CutSmart buffer.

https://nebcloner.neb.com/#!/redigest

Santanu Sasidharan

BamH1 shows star activity according to NEB. I would like to know if anyone has performed the same. @DominiqueLiger

Hi again,

Star activity may be avoided by using HF enzymes. Also you may follow the rules from NEB if no HF enzyme is available:

https://www.neb.com/tools-and-resources/usage-guidelines/star-activity

And it works fine in my experience! Although star activity cloning could work perfectly right even before HF enzymes!!!