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Questions related from Santanu Sasidharan
I have performed fluorescence studies to understand the binding of ligands to my protein. After measuring the fluorescence study and plotting F0/F vs [Q], I found that my plot is linearly...
10 October 2019 570 4 View
I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...
09 September 2019 6,572 6 View
I have a double digestion to perform and i found that the second enzyme is compatible only in cutsmart buffer. Has anyone tried double digestion with BamH1 and Xma1 and cutamart buffer?
06 June 2019 2,926 3 View
I performed a gel extraction of a PCR product and after elution at the last step, the eluted product becomes solidified. I use 0.4% gel for extraction. I do step wise elution of 20+20 ul after...
05 May 2019 5,647 5 View
When I run a PCR with a cloned plasmid, I get three bands ( product of 800 bp, dimeric around 1600 bp and trimeric around 2500 bp). This was for 25 cycles. When I run the same PCR for 35 cycles,...
12 December 2018 2,307 5 View
Do we need to take the hydrogen atoms also into consideration while preparing the gro and itp files? What will be the adverse effect if we don't?
08 August 2018 7,745 1 View
I had used the intrinsic fluorescence of my protein to detect the change in fluorescence intensity with increasing urea concentration. I was astonished to find a huge increase in fluorescence...
08 August 2018 6,614 7 View
I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...
05 May 2017 657 4 View
I have a HPC that has 8 nodes, each node powered by a Xeon 2.5 Ghz and 16 GB ram. I want the GROMACS code where I can split my mdrun amongst the nodes for faster simulation.
05 May 2017 5,882 0 View
Himedia kits suggests drying by maintaining the tubes at 70C for 15 mins. Is this procedure suitable? Will it denature or affect the stability of my plasmid in any way?
08 August 2016 6,237 4 View
I need to study the flagella and external structures. I have some bacteria isolated and need to do SEM analysis of the same. I would like to know if any preparation protocol exists to condition my...
09 September 2014 877 9 View
I had set an autodock run for docking an atp molecule to a kinase enzyme. I had set the number of GA runs to 1000. But, after the dock run had completed and I try to analyze the .dlg file, I am...
01 January 1970 3,325 9 View
