How do I find the Stein Volmer constant?

I have performed fluorescence studies to understand the binding of ligands to my protein. After measuring the fluorescence study and plotting F0/F vs [Q], I found that my plot is linearly...

09 October 2019 486 4 View

Kanamycin resistance cloning into eukaryotic vector?

I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...

08 September 2019 6,458 6 View

Is BamH1(not hf) compatible with cutsmart buffer?

I have a double digestion to perform and i found that the second enzyme is compatible only in cutsmart buffer. Has anyone tried double digestion with BamH1 and Xma1 and cutamart buffer?

05 June 2019 2,845 3 View

Why did the gel eluted product solidify?

I performed a gel extraction of a PCR product and after elution at the last step, the eluted product becomes solidified. I use 0.4% gel for extraction. I do step wise elution of 20+20 ul after...

04 May 2019 5,562 5 View

Why do I get multimeric PCR products?

When I run a PCR with a cloned plasmid, I get three bands ( product of 800 bp, dimeric around 1600 bp and trimeric around 2500 bp). This was for 25 cycles. When I run the same PCR for 35 cycles,...

11 December 2018 2,235 5 View

Why is there an increase in fluorescence intensity of my protein with increase in Urea concentration?

I had used the intrinsic fluorescence of my protein to detect the change in fluorescence intensity with increasing urea concentration. I was astonished to find a huge increase in fluorescence...

07 August 2018 6,499 7 View

Modelling a modified residue in Modeller software

I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...

04 May 2017 563 4 View

GROMACS code for HPC

I have a HPC that has 8 nodes, each node powered by a Xeon 2.5 Ghz and 16 GB ram. I want the GROMACS code where I can split my mdrun amongst the nodes for faster simulation.

04 May 2017 5,791 0 View

I need a method to dry ethanol from centrifuge tubes while performing alkaline lysis method to isolate plasmid?

Himedia kits suggests drying by maintaining the tubes at 70C for 15 mins. Is this procedure suitable? Will it denature or affect the stability of my plasmid in any way?

07 August 2016 6,145 4 View

Can anyone please help me out in preparing my bacterial samples for SEM analysis?

I need to study the flagella and external structures. I have some bacteria isolated and need to do SEM analysis of the same. I would like to know if any preparation protocol exists to condition my...

08 September 2014 775 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View