Himedia kits suggests drying by maintaining the tubes at 70C for 15 mins. Is this procedure suitable? Will it denature or affect the stability of my plasmid in any way?
if this is following a 70% wash of precipitated plasmid DNA then simply leaving at room temp for 20 min will dry the pellet
Dried pelleted DNA if pure will turn clear
to maximise chances of obtaining pure DNA with 70% ethanol removed before air drying then having removed most of the 70% ethanol following precipitation and wash spin pellet for 5 min at 13k; remove most of the ethanol; spin again for 1 minute and remove residual ethanol by using a narrow bore tip ( like a p10 tip).
This will allow your pellet to dry within 20 min at room temp
if you dry DNA pellets at 70C this will aggressively evaporate 70% ethanol but can then over dry the pellet which then makes it difficult to dissolve
if this is following a 70% wash of precipitated plasmid DNA then simply leaving at room temp for 20 min will dry the pellet
Dried pelleted DNA if pure will turn clear
to maximise chances of obtaining pure DNA with 70% ethanol removed before air drying then having removed most of the 70% ethanol following precipitation and wash spin pellet for 5 min at 13k; remove most of the ethanol; spin again for 1 minute and remove residual ethanol by using a narrow bore tip ( like a p10 tip).
This will allow your pellet to dry within 20 min at room temp
if you dry DNA pellets at 70C this will aggressively evaporate 70% ethanol but can then over dry the pellet which then makes it difficult to dissolve