I have a HPC that has 8 nodes, each node powered by a Xeon 2.5 Ghz and 16 GB ram. I want the GROMACS code where I can split my mdrun amongst the nodes for faster simulation.
I have performed fluorescence studies to understand the binding of ligands to my protein. After measuring the fluorescence study and plotting F0/F vs [Q], I found that my plot is linearly...
09 October 2019 621 4 View
I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...
08 September 2019 6,648 6 View
I have a double digestion to perform and i found that the second enzyme is compatible only in cutsmart buffer. Has anyone tried double digestion with BamH1 and Xma1 and cutamart buffer?
05 June 2019 2,978 3 View
I performed a gel extraction of a PCR product and after elution at the last step, the eluted product becomes solidified. I use 0.4% gel for extraction. I do step wise elution of 20+20 ul after...
04 May 2019 5,704 5 View
When I run a PCR with a cloned plasmid, I get three bands ( product of 800 bp, dimeric around 1600 bp and trimeric around 2500 bp). This was for 25 cycles. When I run the same PCR for 35 cycles,...
11 December 2018 2,360 5 View
I had used the intrinsic fluorescence of my protein to detect the change in fluorescence intensity with increasing urea concentration. I was astonished to find a huge increase in fluorescence...
07 August 2018 6,680 7 View
Do we need to take the hydrogen atoms also into consideration while preparing the gro and itp files? What will be the adverse effect if we don't?
07 August 2018 7,802 1 View
I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...
04 May 2017 713 4 View
Himedia kits suggests drying by maintaining the tubes at 70C for 15 mins. Is this procedure suitable? Will it denature or affect the stability of my plasmid in any way?
07 August 2016 6,280 4 View
I need to study the flagella and external structures. I have some bacteria isolated and need to do SEM analysis of the same. I would like to know if any preparation protocol exists to condition my...
08 September 2014 927 9 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View