How do I find the Stein Volmer constant?

I have performed fluorescence studies to understand the binding of ligands to my protein. After measuring the fluorescence study and plotting F0/F vs [Q], I found that my plot is linearly...

09 October 2019 486 4 View

Kanamycin resistance cloning into eukaryotic vector?

I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...

08 September 2019 6,458 6 View

Is BamH1(not hf) compatible with cutsmart buffer?

I have a double digestion to perform and i found that the second enzyme is compatible only in cutsmart buffer. Has anyone tried double digestion with BamH1 and Xma1 and cutamart buffer?

05 June 2019 2,845 3 View

Why did the gel eluted product solidify?

I performed a gel extraction of a PCR product and after elution at the last step, the eluted product becomes solidified. I use 0.4% gel for extraction. I do step wise elution of 20+20 ul after...

04 May 2019 5,562 5 View

Why do I get multimeric PCR products?

When I run a PCR with a cloned plasmid, I get three bands ( product of 800 bp, dimeric around 1600 bp and trimeric around 2500 bp). This was for 25 cycles. When I run the same PCR for 35 cycles,...

11 December 2018 2,235 5 View

Why is there an increase in fluorescence intensity of my protein with increase in Urea concentration?

I had used the intrinsic fluorescence of my protein to detect the change in fluorescence intensity with increasing urea concentration. I was astonished to find a huge increase in fluorescence...

07 August 2018 6,499 7 View

Does hydrogen atoms of ligand necessary for GROMACS simulation?

Do we need to take the hydrogen atoms also into consideration while preparing the gro and itp files? What will be the adverse effect if we don't?

07 August 2018 7,650 1 View

Modelling a modified residue in Modeller software

I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...

04 May 2017 563 4 View

I need a method to dry ethanol from centrifuge tubes while performing alkaline lysis method to isolate plasmid?

Himedia kits suggests drying by maintaining the tubes at 70C for 15 mins. Is this procedure suitable? Will it denature or affect the stability of my plasmid in any way?

07 August 2016 6,145 4 View

Can anyone please help me out in preparing my bacterial samples for SEM analysis?

I need to study the flagella and external structures. I have some bacteria isolated and need to do SEM analysis of the same. I would like to know if any preparation protocol exists to condition my...

08 September 2014 775 9 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View