Is anyone else having issues detecting INDELS using the Gene Studio S5 and Ion Reporter system?

16 March 2022 0 720 Report

We are currently validating the Oncomine Comprehensive Assay Plus in our lab and are struggling to optimize the pipeline for INDEL detection. With it's current settings we are detecting

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Science method

More Jennifer Crow's questions See All

How do I identify And count the number of active sites of an adsorbent?

Jarosite is obtained from bioleaching residue and I want to determine if there are increased active sites biogenic jarosite compared to chemical synthetic jarosite.

10 July 2024 8,132 2 View

What scale can I use to measure consumer's trust with marketing activities?

Scales?

10 July 2024 1,176 2 View

Hello, could you please share how you measure biomass/growth of Aspergillus oryzae in your lab?

Hi, I would like to gain an insight on the various methods of measuring biomass and growth of Aspergillus oryzae, which you have personally tried and would gladly recommend. Thanks.

08 July 2024 6,057 2 View

Any automatic way to outline an image to measure the area in pixels?

Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...

27 June 2024 5,068 1 View

What is the most pressing agricultural setback to post harvest produce?

The major causes of post harvest loss of produce in Ghana.

20 June 2024 6,445 5 View

Suitable antibodies for HPV16 E6/E7 in western blot?

Hi, I want to analyze the protein expression levels of HPV16 E6/E7 in the cervical cancer cell lines SiHa and CaSki after treatment with silencing RNA for this oncogene. Can anyone recommend a...

03 June 2024 9,579 3 View

Is there a non-toxic dye to visualize cannula placement in mouse brain?

I'm implanting Alzet pumps in mice with a brain infusion cannula to deliver into the ICV space. I am familiar with the technique that includes removing the pump from the tubing and then using a...

22 May 2024 7,121 3 View

My CONTCAR, WAVECAR, and CHGCAR files are generated empty after an optimization calculation in VASP. What am I doing wrong?

Hi, I am performing optimization calculations in VASP for oxides. After my calculation was finished, I checked the OUTCAR file, and the optimization finished well. But when I checked the other...

29 April 2024 1,506 2 View

Which non-parametric test do I need to use?

Hello, I have data in SPSS from a scale I did, I have 1 variable which is age (separated into 3 categories using values of 1-3), a 2nd variable which is gender (again separated into 3 categories...

19 April 2024 9,983 3 View

How would you prepare red blood cell samples for an ELISA?

We are trying to use the Life Diagnostics Trout HSP70-23 to look at Heat Shock Protein 70 in fish red blood cells to look at physiological changes in response to stressors. I have found very...

08 April 2024 1,134 0 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

What is human-computer interaction (HCI)?

22 July 2024 10,056 2 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

How to determine the position of occupancy of the dopant? - whether it is doped in tetrahedral or octahedral site?

Suppose a material "A" has both tetrahedral and octahedral sites and we are doping another material "B" - usually an ion into it. How can we detect if the dopant has occupied the octahedral site...

17 July 2024 4,299 4 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View