Tissues are mostly fresh frozen.
I have given one of my papers to publish in a journal. That was published and after the publication of that article, they again submit that article to a different publication without taking my...
13 June 2023 9,277 7 View
By mistake, my previously uploaded article was deleted and my research interest score also became deleted with that deleted one. Anyone, please guide me on how could i restore the previous one.
03 June 2023 3,759 0 View
I am an engineer trying to understand breast cancer stem cells. I understand breast cancer can be classified according to its molecular signatures e.g. HER2+ or TNBC. Do these classifications...
28 February 2022 383 1 View
I wish to understand whether it is possible to make a phonon mode resonate with an externally applied signal in a lab, and whether a laser can be used for the purpose (if not, is there any other...
18 October 2021 7,842 3 View
I have a soluble yeast protein that needs to be transported to mitchondria for processing. I have put the Cox IV presequence infront of the ORF. I know it would be synthsized in cytosol, imported...
01 February 2021 3,018 3 View
As a retired person, I am contemplating for a DNA Diagnostic Kit with stable DNA for Field applications. Which of the following is a better option and is there a better option. I have solution for...
07 January 2021 8,883 2 View
How to construct a heterostructure in Quantum Espresso for e.g: GaAs/GaN?
01 March 2020 7,984 8 View
What will be the strategy to publish the learning experiences of development and deployment of mobile phone based agro-advisory system in reputed journal? Should I give more stress on my...
14 October 2018 3,296 0 View
Aucomea klaineaena is an african tree used for its timber However I have observed that the sometimes the log cracks open immediately after cutting the tree. this happens throughout the year ( so...
25 June 2018 3,528 3 View
We are working on induction heating and we are having trouble to measure the high frequency magnetic field strength (about 1 kA/m)? We have the coil design (nos. of turns and dia) and we know the...
15 November 2015 3,541 9 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
