We designed siRNA for Rabies virus silencing, I need to know the siRNA may cause negative effect of any protein mRNA in mice or not, so before giving this siRNA to mice I need to know. So, please suggest me any softwate available for this.
You could use this http://sivirus.rnai.jp/ However, please be advised that it aims to target highly conserved regions that contain "constrained" elements. I think you could check on the specific effect based on the repeat sequences or translated/AT/GC composition.