Ficoll is neutral, highly branched, hydrophilic polymer used for the seperation on the density gradient. is there any other option for the seperation of bone marrow derived stem cell.
Hello Muhammad Fozan
You may want to try Percoll as an alternative. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.
Moreover, Percoll is a low viscosity density gradient medium for preparation of cells. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 x g). It preserves the viability and morphological integrity of cells.
You could refer to the paper attached below. It may be helpful.
Article Isolation of Bone Marrow-Derived Stem Cells using Density-Gr...
Best.
Ficoll is a commonly used reagent for the separation of bone marrow-derived stem cells on a density gradient. However, there are other options for stem cell separation as well. Some other options include:
It's important to note that the choice of method will depend on the research goals, the availability of resources, and the characteristics of the sample. Additionally, some methods may be more appropriate for certain types of stem cells, or for certain applications, like cell therapy.
You could use LymphoPrep from Stem Cell Technologies. I have using it to isolate MNCs from BMs of non human primates and it has been working good for us.
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