Spencer G. Smith

3 Questions 1 Answers 0 Followers

Questions related from Spencer G. Smith

Phosphomimetic Peptides & Phospho-Specific Antibodies?

I am performing experiments using synthetic cell-penetrating peptides in acute hippocampal slices. Our lab has verified the NLS-mediated nuclear delivery of a number of such peptides in the region...

28 December 2019 8,060 2 View

What is the recommended fixation time of 400 um hippocampal slices in 4% PFA?

I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...

23 June 2018 9,719 5 View

Is agitation necessary during IHC antibody incubation for 400 um hippocampal slices?

I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid...

10 June 2018 9,737 3 View