In our lab, Sanyo-MCO-175 water jacket Co2 incubator was used for cell culture maintenance. Past 3 month we were faced contamination problem in our cell lines (A549 and MCF7). So I was checked all possibility way to prevent contamination but I didn’t eradicate bacteria (rod and spherical shaped).
1. I was checked Co2 cylinder and Co2 incubator connection, no external filter attachment. If I will insert 0.22µ filter means, there is a possibility to remove bacterial contamination.
2. Sanyo-MCO-175 water jacket Co2 incubator contain in build attachment of 0.3µ filter.
3. Please suggest me to eradicate those problems.