Ian Teo

3 Questions 225 Answers 0 Followers

Questions related from Ian Teo

Has anyone made their own RT for use in real-time RT-PCR?

The main cost of performing RT-PCR is that of doing the reverse transcription. Whilst the price of Taq has come down substantially, the price of recombinant reverse transcriptase has not and no...

05 May 2014 5,537 20 View

How reliable is your PCR machine ? -has anyone had any negative experiences with a 24 tube Piko thermocycler?

We have had 2 failures with a Piko thermocycler in the last 4 years which seems a high failure rate. The apparent cost of repairing the machine is 2/3 of its brand new cost with labour charges of...

03 March 2014 3,652 0 View

Does anyone have experience with a Genie Vortex Disrupter for RNA extraction?

I am looking for an affordable method of homogenisation of tissue (gut) for RNA extraction. Bead beaters are off our budget. The Genie Vortex disrupter claims to do much the same but at a fraction...

02 February 2014 4,144 0 View