I analyzed particulate matter (PM) sample from Selam industrial area, Tamil Nadu, India. I added my particulate matter in various concentration to the A549 cells. I used 96 well plates. After 16 hours of exposure, I removed supernatant to store -20 ˚c for my Cytokine and Chemokine assays and then the cells were used MTT assay.
(1). Without ELISA KITs? If it possible to do that assays?
(2). I want protocol for Cytokine and Chemokine detection Assays.
(3). If i will use PM10 treated A549 medium supernatant for cytokine and chemokine detection or cell pellet?
(4). Please suggest any other method for PM toxicity measurement?
(5). ELISA KIT is available in market please suggest best one?