What is the optimum storage condition for FBS after it has been aliquoted in 50ml ? why?

Dear Research friends, i have one doubt about FBS (fetal bovine serum) storage condition?.. Once you are all heat inactivated your serum and then aliquote 50 ml each to store at -20C . Why? Once...

10 November 2014 565 8 View

No title

In our lab, Sanyo-MCO-175 water jacket Co2 incubator was used for cell culture maintenance. Past 3 month we were faced contamination problem in our cell lines (A549 and MCF7). So I was checked all...

07 August 2014 7,482 8 View

How can I identify FBS Contamination?

I have doubt about our lab south american FBS because it will be expired march 2015. I have a doubt about FBS also. In my A549 culture this one gave rad and spiral shape contamination. So can you...

06 July 2014 1,558 19 View

Could anyone help me, PCR reaction mixture related doubts?

I used GENET BIO (ExPrime Tag Premix (2x)) for my PCR rxn. It contains: DNA Polymerase 1 Unit/10 microliter, 20 mM Tris-HCL, 80 mM KCl, 4mM Mgcl2, enzyme stabilizer, sediment loading dye, pH- 9.0...

09 October 2013 9,396 4 View

Can someone advise on ligation related queries?

I didn't get my clone (pcDNA3.1+insert+GFP). I cloned GFP successfully but my promoter cloning is very difficult . I replaced CMV promoter with my promoter (2 different promoter (1).930 BP and...

07 August 2013 4,454 1 View

Stable cell line maintenance related doubts

I have pcDNA3.1+ Insert + GFP vector. I transfected this vector into A549 cell line. I got minimum GFP expression after 48 hours (only 5 to 10 cells give GFP fluorescence) and I trypsinized that...

06 July 2013 2,056 12 View

Is passage number important for publication?

i have one more doubts.. we were maintained A549, MCF7, INS cell lines if i should maintain upto 50 passage or more. 1). Normally How many passage cells are used MTT or some other assay? 2). If we...

06 July 2013 9,170 13 View

MTT assay related doubts?

06 July 2013 8,687 13 View

No title

I analyzed particulate matter (PM) sample from Selam industrial area, Tamil Nadu, India. I added my particulate matter in various concentration to the A549 cells. I used 96 well plates. After 16...

06 July 2013 7,475 0 View

Passage number is important for following assays?

1). Wound healing 2). Cell proliferation 3). Apoptosis 4). Etc... I have A549, MCF7, HepG2, THP1 . So please suggest to me, which passage number is suitable for above assays? My work :...

06 July 2013 5,388 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View