Can anyone help with my mouse aortic ring assay related doubts?

More Dr. Sankareswaran Senthil Kumar's questions See All

What is the optimum storage condition for FBS after it has been aliquoted in 50ml ? why?

Dear Research friends, i have one doubt about FBS (fetal bovine serum) storage condition?.. Once you are all heat inactivated your serum and then aliquote 50 ml each to store at -20C . Why? Once...

10 November 2014 699 8 View

Is a 0.22µ filter essential in-between Co2 cylinder and Co2 incubator connection?

In our lab, Sanyo-MCO-175 water jacket Co2 incubator was used for cell culture maintenance. Past 3 month we were faced contamination problem in our cell lines (A549 and MCF7). So I was checked all...

07 August 2014 7,581 8 View

How can I identify FBS Contamination?

I have doubt about our lab south american FBS because it will be expired march 2015. I have a doubt about FBS also. In my A549 culture this one gave rad and spiral shape contamination. So can you...

06 July 2014 1,939 19 View

Could anyone help me, PCR reaction mixture related doubts?

I used GENET BIO (ExPrime Tag Premix (2x)) for my PCR rxn. It contains: DNA Polymerase 1 Unit/10 microliter, 20 mM Tris-HCL, 80 mM KCl, 4mM Mgcl2, enzyme stabilizer, sediment loading dye, pH- 9.0...

09 October 2013 9,502 4 View

Can someone advise on ligation related queries?

I didn't get my clone (pcDNA3.1+insert+GFP). I cloned GFP successfully but my promoter cloning is very difficult . I replaced CMV promoter with my promoter (2 different promoter (1).930 BP and...

07 August 2013 4,581 1 View

Stable cell line maintenance related doubts

I have pcDNA3.1+ Insert + GFP vector. I transfected this vector into A549 cell line. I got minimum GFP expression after 48 hours (only 5 to 10 cells give GFP fluorescence) and I trypsinized that...

06 July 2013 2,209 12 View

Is passage number important for publication?

i have one more doubts.. we were maintained A549, MCF7, INS cell lines if i should maintain upto 50 passage or more. 1). Normally How many passage cells are used MTT or some other assay? 2). If we...

06 July 2013 9,304 13 View

Cytokines (IL 6, IL-1β, TNF-a) and Chemokine (MIP-2) Assay protocols related queries?

I analyzed particulate matter (PM) sample from Selam industrial area, Tamil Nadu, India. I added my particulate matter in various concentration to the A549 cells. I used 96 well plates. After 16...

06 July 2013 7,572 0 View

MTT assay related doubts?

I was used different concentrations (100, 50, 25, 12.5, 6.25, 3.125 µg/ml) of particulate matter (PM10) treated with A549 cell line for 24 hours, 48 hours. I was completed 24 hours reading at 570...

06 July 2013 8,797 13 View

Passage number is important for following assays?

1). Wound healing 2). Cell proliferation 3). Apoptosis 4). Etc... I have A549, MCF7, HepG2, THP1 . So please suggest to me, which passage number is suitable for above assays? My work :...

06 July 2013 5,520 12 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

Giorgio Seano

Why agarose and not matrigel (basement membrane rich matrix) for example? I did a lot of them in matrigel... it is pretty easy!

In case you can also use some of the gels of BD (look at Hydrogel for ex) (http://www.bdbiosciences.com/cellculture/ecm/ecmtypes/other.jsp)

In attachment an article that can help you!

Article Modeling human tumor angiogenesis in a three-dimensional cul...

Dr. Sankareswaran Senthil Kumar

Dr Giorgio Seano thank you very much for your suggestion and guidelines.

I forgot... maybe you can find the information you want in this method article!

they do that in agarose... I have never tried!

Article Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model