I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Has anyone used an inertial navigation system with RTK (Real Time Kinematic)?

INS-RTK modules claim to be close to a cm accuracy, has anyone used one and if so, how expensive can the telematic charges be if I have a case to run it 18h per day?

14 December 2020 6,139 8 View

What are first, second and third cracks (red, green&blue color outline) in crack pattern of RC beams in ANSYS APDL?

Hello everyone, Can anyone kindly explain what is mean by three different colours of cracks in RC beams in ANSYS APDL and the features of first crack (red colour outline) second crack (green...

17 November 2020 2,512 1 View

What should be reason for higher values of Ultimate results for RC beam simulations in ANSYS APDL?

My RC beams experimental ultimate load & deflection was 97kN and 20mm resp. But when I model it in ansys apdl it run continuously up to a load of 140kN and 60mm deflection and still...

02 October 2020 8,747 2 View

Why the solution runs continuously even after reaching ultimate strength of beam in ANSYS APDL?

I am analyzing RC beam in APDL and tried convergence criteria with F=0.05 once then U=0.05 and also tried both criterias simultaneously but what I found is my solution continuous even upto twice...

02 October 2020 1,490 2 View

Need "E.Hognestad;" compressive stress-strain curve explanation?

Can anyone kindly explain in case of Hognestad compressive stress-strain curve for concrete, they have given fc" =0.85fc', but why researchers are use fc"=fc' instead. Where fc" =ultimate...

14 August 2020 7,508 3 View

How to perform Molecular dynamics (MD) simulation for corrosion system ?

Dear researchers, Greetings! Please help me to study surface of Fe(110) with Organic Corrosion inhibitor molecules using Materials Studio software. Kindly give suggestion to create a simulation...

02 August 2020 4,267 2 View

How to measure porosity/diameter of electrospun nanofibers using imagej?

Hi all, I have SEM images of electrospun nanofibers and I'd like to measure its porosity/diameter. However I'm not sure how to do it with ImageJ or any other software. Can anybody help me please?...

27 July 2020 5,925 7 View

How important is it to check the psychometric properties of translated version of a gold standard scale?

There is a scale in English that has well established psychometric properties. I want to use the translated version of the scale in my study. Do I have to test all the psychometric properties of...

11 June 2020 3,951 14 View

What characterization method is used for realizing different phases in a micron size particle?

12 March 2020 6,331 14 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View