Is there easy and reliable online software for estimation of RNA-protein binding efficiency?

Hello Everyone, I would like to check if my protein of interest can bind to RNA of interest. Before moving forward I would like to check using any available prediction software. Please let me...

11 December 2019 6,137 3 View

How much RNA typically we can get from single cell?

How much RNA typically we can get RNA from any single cell? Has anyone isolated RNA from single cells? Using which method or protocol? Based on your experience, what possible downstream assays can...

06 July 2018 5,118 8 View

What are exosome negative markers?

Hi, I am relatively new to the exosome/MV study. I would like to know which proteins and RNAs are absent (or likely to be absent) in exosomes/MV (negative control). I am aware about the...

11 December 2017 3,116 5 View

Is it possible to see eukaryotic cells with attached fluorescent-bacteria in FACS analysis?

11 December 2016 6,773 5 View

Is "Cumulative Thesis" recognized everywhere?

02 March 2016 2,563 0 View

What is the best method to obtain single cell suspension of BCG?

I am looking for protocol or methodology to obtain single cell suspension of M. bovis BCG. Normally they occur in clumps when I see them under microscope. I found some methods like Sonication,...

11 December 2015 9,391 3 View

Is there any database or online search tools available to find genes/chromosomes which are targeted by known long non coding RNA?

Finding Long noncoding RNA targets.

07 August 2014 5,036 5 View

How to intersect more than 10 non numerical data sets?

I have more than 10 sets of non numerical data and want to find out common element present in all sets. How can I achieve this, or which software is helpful?

10 November 2013 5,302 7 View

Can exosomal content (RNA, DNA or protein) reach endosomes and their receptors?

I am trying to find review or publication that can answer the question. Literature says, Exosomal content can be poured into the cell cytoplasm. But I was wondering if Exosomal content is also...

01 January 1970 1,183 0 View

What is the shortest nucleotide sequence we can map with confidence?

I have a NGS RNA seq data and would like to search RNAs and their expression profile. I would like to know what is the shortest RNA (shorter than miRNAs) that can be identified as a RNA species....

01 January 1970 8,992 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View