Is there easy and reliable online software for estimation of RNA-protein binding efficiency?

Hello Everyone, I would like to check if my protein of interest can bind to RNA of interest. Before moving forward I would like to check using any available prediction software. Please let me...

11 December 2019 6,137 3 View

How much RNA typically we can get from single cell?

How much RNA typically we can get RNA from any single cell? Has anyone isolated RNA from single cells? Using which method or protocol? Based on your experience, what possible downstream assays can...

06 July 2018 5,118 8 View

What are exosome negative markers?

Hi, I am relatively new to the exosome/MV study. I would like to know which proteins and RNAs are absent (or likely to be absent) in exosomes/MV (negative control). I am aware about the...

11 December 2017 3,116 5 View

Is it possible to see eukaryotic cells with attached fluorescent-bacteria in FACS analysis?

11 December 2016 6,773 5 View

What is the best method to obtain single cell suspension of BCG?

I am looking for protocol or methodology to obtain single cell suspension of M. bovis BCG. Normally they occur in clumps when I see them under microscope. I found some methods like Sonication,...

11 December 2015 9,391 3 View

Is there any database or online search tools available to find genes/chromosomes which are targeted by known long non coding RNA?

Finding Long noncoding RNA targets.

07 August 2014 5,036 5 View

In target prediction, what is the basis for miRmap score selection?

As you can see in screenshot final miRmap score is 80.06, but in the explanation section it gives two target sites with two different miRmap scores (55.50 and 75.51). How is the final score...

04 May 2014 2,355 0 View

How to intersect more than 10 non numerical data sets?

I have more than 10 sets of non numerical data and want to find out common element present in all sets. How can I achieve this, or which software is helpful?

10 November 2013 5,302 7 View

Can exosomal content (RNA, DNA or protein) reach endosomes and their receptors?

I am trying to find review or publication that can answer the question. Literature says, Exosomal content can be poured into the cell cytoplasm. But I was wondering if Exosomal content is also...

01 January 1970 1,183 0 View

What is the shortest nucleotide sequence we can map with confidence?

I have a NGS RNA seq data and would like to search RNAs and their expression profile. I would like to know what is the shortest RNA (shorter than miRNAs) that can be identified as a RNA species....

01 January 1970 8,992 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View