Is there easy and reliable online software for estimation of RNA-protein binding efficiency?

Hello Everyone, I would like to check if my protein of interest can bind to RNA of interest. Before moving forward I would like to check using any available prediction software. Please let me...

11 December 2019 6,137 3 View

How much RNA typically we can get from single cell?

How much RNA typically we can get RNA from any single cell? Has anyone isolated RNA from single cells? Using which method or protocol? Based on your experience, what possible downstream assays can...

06 July 2018 5,118 8 View

What are exosome negative markers?

Hi, I am relatively new to the exosome/MV study. I would like to know which proteins and RNAs are absent (or likely to be absent) in exosomes/MV (negative control). I am aware about the...

11 December 2017 3,116 5 View

Is it possible to see eukaryotic cells with attached fluorescent-bacteria in FACS analysis?

11 December 2016 6,773 5 View

Is "Cumulative Thesis" recognized everywhere?

02 March 2016 2,563 0 View

What is the best method to obtain single cell suspension of BCG?

I am looking for protocol or methodology to obtain single cell suspension of M. bovis BCG. Normally they occur in clumps when I see them under microscope. I found some methods like Sonication,...

11 December 2015 9,391 3 View

In target prediction, what is the basis for miRmap score selection?

As you can see in screenshot final miRmap score is 80.06, but in the explanation section it gives two target sites with two different miRmap scores (55.50 and 75.51). How is the final score...

04 May 2014 2,355 0 View

How to intersect more than 10 non numerical data sets?

I have more than 10 sets of non numerical data and want to find out common element present in all sets. How can I achieve this, or which software is helpful?

10 November 2013 5,302 7 View

Can exosomal content (RNA, DNA or protein) reach endosomes and their receptors?

I am trying to find review or publication that can answer the question. Literature says, Exosomal content can be poured into the cell cytoplasm. But I was wondering if Exosomal content is also...

01 January 1970 1,183 0 View

What is the shortest nucleotide sequence we can map with confidence?

I have a NGS RNA seq data and would like to search RNAs and their expression profile. I would like to know what is the shortest RNA (shorter than miRNAs) that can be identified as a RNA species....

01 January 1970 8,992 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View