I use the CBS Scientific gel assembly apparatus (http://www.cbsscientific.com/verticalmini-gelelectrophoresissystemaccessories.aspx) to make polyacrylamide gels. Lately, I have been experiencing a lot of wells collapsing such that the walls fall over and it is nearly impossible to get them upright again.
The slanted, fallen over well walls cause sample to be loaded at an angle which results in samples overlapping in the resolving gel. This issue has made interpreting gels very hard as individual lanes become nearly impossible to discern.
Does anyone know how to prevent the well walls from collapsing? Any technical advice would be helpful and appreciated.